Patton Oswalt Uncle Ben (voice)

Jim Cummings Hammerhead (voice)

Nadji Jeter Miles Morales (voice)

Alastair Duncan Adrian Toomes (voice)

Robbie Daymond Peter Parker / Spider-Man (voice)

John DiMaggio Raymond Warren (voice)

Nancy Linari Aunt May (voice)

Max Mittelman Thug #2 / Little Kid (voice)

Melanie Minichino Helicopter Pilot (voice)

Ben Diskin Flash Thompson (voice)

Scott Menville Grady Scraps (voice)

Josh Keaton Unidentified Voice / Police Officer (voice)

Fred Tatasciore Trucker/Mr. Slott (voice)

Laura Bailey Osborn Recruiter #2 (voice)

Jason Spisak Alistair Smythe (voice)

Aaron Abrams Tinkerer / Old Man (voice)

Sofia Carson Keemia (voice)

Yuri Lowenthal Nocturnal (voice)

Bob Joles J. Jonah Jameson (voice)

Zeno Robinson Randy Robertson (voice)

Matthew Mercer Aleksei Sytsevich/Comic Book Guy (voice)

Grey DeLisle Black Cat (voice)

Cameron Boyce Herman Schultz / Shocker (voice)

Nathaniel J. Potvin Prowler (voice)

Richard Doyle Police Officer #3 (voice)

Daniel Hagen Cop (voice)

Travis Willingham Sandman / Flint Marko (voice)

Jeremy Shada Ross Caliban (voice)

Sumalee Montano Yuri Watanabe (voice)

Felicia Day Mary Jane (voice)

Alex Désert Swarm (voice)

Yvette Nicole Brown Head Administrator (voice)

Sol Choi

Philip Pignotti

Dan Duncan

Kevin Shinick

Sterling Gates

J.T. Krul

Mike Fasolo

Kevin Burke

Chris Wyatt